Isolation, characterization and growth assessment of biodegrading chlorpyrifos-methyl Bacillus species isolated from Algerian soil

Chlorpyrifos-methyl (CM) is a broad-spectrum organophosphate insecticide, which widely used in pest control. In this research, the isolation, and biochemical molecular identification of bacterial strains obtained from three soils located northeastern Algeria were carried out, as well evaluation their ability to grow presence CM. Out 48 isolates between Gram-negative Gram-positive identified, several able on mineral agar with at least 25 mg·l–1 Four bacteria showed best growth capacity, identified Bacillus sp. H1-80, Brevibacterium frigoritolerans strain WJB99 two GL5. The tested for liquid media CM sole energy carbon source. general, these slow but significant visualized by 600 nm turbidity control, suggesting that they could be bioremediation applications polluted soils.